Quantification of Platelet-Fibrin Interactions

Platelets are integral to maintaining homeostasis in the human body by interacting with fibrin to form strong and stable clots that prevent blood loss. It is important to visualize these interactions at the nanoscale to better understand platelet-fibrin interactions and the role of glycoprotein receptors in clot formation. The purpose of this study is to quantify platelet fibrin interactions using 4X expansion microscopy (ExM) of platelets and associated receptors. Using ExM techniques, this study aims to assess the distribution of GPIIb/IIIa and FXIII during platelet activation, leading to a greater understanding of internal clot mechanics. This information could have uses in therapeutic strategies for clotting disorders, anticoagulant treatment, and regenerative medicine.